Increased Risk for Venous Thrombosis in Carriers of the Prothrombin G→A²⁰²¹⁰ Gene Variant

Abstract

Background: A mutation in the prothrombin gene (G→A²⁰²¹⁰) has been associated with higher plasma prothrombin levels and an increased tendency for venous thrombosis.

Objective: To determine whether the prothrombin A²⁰²¹⁰ allele is independently associated with the occurrence of venous thrombosis.

Design: Case-control study.

Setting: Two thrombosis centers in southern Italy.

Patients: 281 consecutive patients with venous thrombosis confirmed by objective tests and 850 controls.

Measurements: Medical history was collected on standardized questionnaires. The presence of prothrombin G→A²⁰²¹⁰ and factor V Leiden mutations was determined by polymerase chain reaction. The presence of anticoagulant factors and prothrombin activity was determined by tests of function.

Results: In 150 controls, increased prothrombin activity (P < 0.001) was associated with the prothrombin A²⁰²¹⁰ allele. This allele was more frequent in patients than in controls (8.01% compared with 2.29%; P < 0.001) and was associated with an increased risk for thrombosis (odds ratio, 3.88 [95% CI, 2.23 to 6.74]). The increased prevalence of this allele was independent of the presence of the factor V Leiden mutation. After adjustment for sex, age, arterial thrombosis, and factor V Leiden mutation, the risk was still significantly elevated (odds ratio, 3.13 [CI, 1.89 to 5.21]). Moreover, the overall prevalence of inherited coagulation abnormalities was significantly higher in patients with thrombosis of the lower extremities than in patients with thrombosis of the upper extremities (odds ratio, 3.77 [CI, 1.10 to 12.93]). Fourteen patients carried both the prothrombin G→A²⁰²¹⁰ and factor V Leiden mutations.

Conclusions: The prothrombin A²⁰²¹⁰ allele is independently associated with the occurrence of venous thrombosis, particularly in patients with a history of thrombosis of the lower extremities.